Department of General Practice and Preventive Medicine

Health Sciences University of Mongolia

Department of Anatomy,

Health Sciences University of Mongolia

The goal of our study was to determine the effects of ethanol on the ectodermal and mesodermal organs employing an animal model. After fertilization, the animals were divided into three groups. The first group of rats received 5% ethanol containing diets. On GD 21, the pregnant rats were anesthetized with ether, and their fetuses were removed by cesarean section. For histological observation, sections were stained with hematoxilin and eosin. For immunohistochemical examination, sections were prepared with anti-laminin and anti-nestin antibodies using an immunoperoxidase method. Nestin was noted to be distributed abundantly in the granular layer of the epidermis. Our study suggested that the

development of skin and its derivates was delayed, and the apoptotic processes were retarded from chronic ethanol exposure.

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